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排序方式: 共有967条查询结果,搜索用时 31 毫秒
51.
Djoere Gaublomme Tom Buyens Lies De Groef Michelle Stakenborg Els Janssens Signe Ingvarsen Astrid Porse Niels Behrendt Lieve Moons 《Journal of neurochemistry》2014,129(6):966-979
Restoration of correct neural activity following central nervous system (CNS) damage requires the replacement of degenerated axons with newly outgrowing, functional axons. Unfortunately, spontaneous regeneration is largely lacking in the adult mammalian CNS. In order to establish successful regenerative therapies, an improved understanding of axonal outgrowth and the various molecules influencing it, is highly needed. Matrix metalloproteinases (MMPs) constitute a family of zinc‐dependent proteases that were sporadically reported to influence axon outgrowth. Using an ex vivo retinal explant model, we were able to show that broad‐spectrum MMP inhibition reduces axon outgrowth of mouse retinal ganglion cells (RGCs), implicating MMPs as beneficial factors in axonal regeneration. Additional studies, using more specific MMP inhibitors and MMP‐deficient mice, disclosed that both MMP‐2 and MT1‐MMP, but not MMP‐9, are involved in this process. Furthermore, administration of a novel antibody to MT1‐MMP that selectively blocks pro‐MMP‐2 activation revealed a functional co‐involvement of these proteinases in determining RGC axon outgrowth. Subsequent immunostainings showed expression of both MMP‐2 and MT1‐MMP in RGC axons and glial cells. Finally, results from combined inhibition of MMP‐2 and β1‐integrin were suggestive for a functional interaction between these molecules. Overall, our data indicate MMP‐2 and MT1‐MMP as promising axonal outgrowth‐promoting molecules.
52.
Tanzeela Awan Aaron Babendreyer Abid Mahmood Alvi Stefan Düsterhöft Daniela Lambertz Jörg W. Bartsch Christian Liedtke Andreas Ludwig 《Journal of cellular and molecular medicine》2021,25(4):1982-1999
Hepatocellular carcinoma (HCC) is one of the most common metastatic tumours. Tumour growth and metastasis depend on the induction of cell proliferation and migration by various mediators. Here, we report that the A Disintegrin and Metalloproteinase (ADAM) 8 is highly expressed in murine HCC tissues as well as in murine and human hepatoma cell lines Hepa1-6 and HepG2, respectively. To establish a dose-dependent role of different ADAM8 expression levels for HCC progression, ADAM8 expression was either reduced via shRNA- or siRNA-mediated knockdown or increased by using a retroviral overexpression vector. These two complementary approaches revealed that ADAM8 expression levels correlated positively with proliferation, clonogenicity, migration and matrix invasion and negatively with apoptosis of hepatoma cells. Furthermore, the analysis of pro-migratory and proliferative signalling pathways revealed that ADAM8 expression level was positively associated with expression of β1 integrin as well as with the activation of focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), Src kinase and Rho A GTPase. Finally, up-regulation of promigatory signalling and cell migration was also seen with a proteolytically inactive ADAM8 mutant. These findings reveal that ADAM8 is critically up-regulated in hepatoma cells contributes to cell proliferation and survival and furthermore induces pro-migratory signalling pathways independently of its proteolytic activity. By this, ADAM8 can promote cell functions most relevant for HCC growth and metastasis. 相似文献
53.
Alexandra Hauser-Kawaguchi Cornelia Tolg Teresa Peart Mark Milne Eva A. Turley Leonard G. Luyt 《Bioorganic & medicinal chemistry》2018,26(18):5194-5203
The receptor for hyaluronan mediated motility (RHAMM, gene name HMMR) belongs to a group of proteins that bind to hyaluronan (HA), a high-molecular weight anionic polysaccharide that has pro-angiogenic and inflammatory properties when fragmented. We propose to use a chemically synthesized, truncated version of the protein (706–767), 7?kDa RHAMM, as a target receptor in the screening of novel peptide-based therapeutic agents. Chemical synthesis by Fmoc-based solid-phase peptide synthesis, and optimization using pseudoprolines, results in RHAMM protein of higher purity and yield than synthesis by recombinant protein production. 7?kDa RHAMM was evaluated for its secondary structure, ability to bind the native ligand, HA, and its bioactivity. This 62-amino acid polypeptide replicates the HA binding properties of both native and recombinant RHAMM protein. Furthermore, tubulin-derived HA peptide analogues that bind to recombinant RHAMM and were previously reported to compete with HA for interactions with RHAMM, bind with a similar affinity and specificity to the 7?kDa RHAMM. Therefore, in terms of its key binding properties, the 7?kDa RHAMM mini-protein is a suitable replacement for the full-length recombinant protein. 相似文献
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56.
目的:探讨阿托伐他汀强化降脂治疗急性脑梗死(ACI)的临床疗效,并分析其对肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)、白细胞介素-18(IL-18)及基质金属蛋白酶-9(MMP-9)水平的影响。方法:选取2015年3月-2016年12月我院收治的82例ACI患者,采用随机数字表法随机分为强化组(n=41)与常规组(n=41)。在常规治疗的基础上,常规组患者给予20 mg/次的阿托伐他汀治疗,强化组患者给予40 mg/次的阿托伐他汀治疗,两组均连续治疗8w。治疗结束后对比两组患者的临床疗效,对比两组患者治疗前后总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、组织型纤溶酶原激活物(t-PA)、血浆纤溶酶原激活物抑制剂-1(PAI-1)、血浆纤维蛋白原(FIB)、TNF-α、IL-10、IL-18、MMP-9水平。结果:强化组与常规组患者的总有效率分别为95.12%、80.49%,与常规组对比,强化组患者的临床总有效率明显升高(P0.05);两组患者治疗后的TC、TG、LDL-C、PAI-1、FIB、TNF-α、IL-18、MMP-9水平均较治疗前显著降低,HDL-C、t-PA、IL-10水平均显著升高(P0.05),且治疗后强化组患者的TC、TG、LDL-C、PAI-1、FIB、TNF-α、IL-18、MMP-9水平均低于常规组,HDL-C、t-PA、IL-10均高于常规组(P0.05)。结论:阿托伐他汀强化降脂治疗ACI疗效较好,能够显著改善患者血脂、纤溶系统及炎症因子相关指标水平,具有降脂、调节纤溶活性及抑制炎症的作用。 相似文献
57.
Mohd M. Khan Orna Ernst Jing Sun Iain D.C. Fraser Robert K. Ernst David R. Goodlett Aleksandra Nita-Lazar 《Journal of molecular biology》2018,430(17):2641-2660
One cause of sepsis is systemic maladaptive immune response of the host to bacteria and specifically, to Gram-negative bacterial outer-membrane glycolipid lipopolysaccharide (LPS). On the host myeloid cell surface, proinflammatory LPS activates the innate immune system via Toll-like receptor-4/myeloid differentiation factor-2 complex. Intracellularly, LPS is also sensed by the noncanonical inflammasome through caspase-11 in mice and 4/5 in humans. The minimal functional determinant for innate immune activation is the membrane anchor of LPS called lipid A. Even subtle modifications to the lipid A scaffold can enable, diminish, or abolish immune activation. Bacteria are known to modify their LPS structure during environmental stress and infection of hosts to alter cellular immune phenotypes. In this review, we describe how mass spectrometry-based structural analysis of endotoxin helped uncover major determinations of molecular pathogenesis. Through characterization of LPS modifications, we now better understand resistance to antibiotics and cationic antimicrobial peptides, as well as how the environment impacts overall endotoxin structure. In addition, mass spectrometry-based systems immunoproteomics approaches can assist in elucidating the immune response against LPS. Many regulatory proteins have been characterized through proteomics and global/targeted analysis of protein modifications, enabling the discovery and characterization of novel endotoxin-mediated protein translational modifications. 相似文献
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59.
Daniela Ligi Sonia Benitez Lidia Croce Andrea Rivas-Urbina Núria Puig Jordi Ordóñez-Llanos Ferdinando Mannello Jose Luis Sanchez-Quesada 《生物化学与生物物理学报:疾病的分子基础》2018,1864(12):3559-3567
Objective
Electronegative LDL (LDL(?)) is involved in atherosclerosis through the activation of the TLR4/CD14 inflammatory pathway in monocytes. Matrix metalloproteinases (MMP) and their inhibitors (tissue inhibitors of metalloproteinase [TIMP]) are also crucially involved in atherosclerosis, but their modulation by LDL(?) has never been investigated. The aim of this study was to examine the ability of LDL(?) to release MMPs and TIMPs in human monocytes and to determine whether sulodexide (SDX), a glycosaminoglycan-based drug, was able to affect their secretion.Approach and results
Native LDL (LDL(+)) and LDL(?) separated by anion-exchange chromatography were added to THP1-CD14 monocytes in the presence or absence of SDX for 24?h. A panel of 9 MMPs and 4 TIMPs was analyzed in cell supernatants with multiplex immunoassays. The gelatinolytic activity of MMP-9 was assessed by gelatin zymography. LDL(?) stimulated the release of MMP-9 (13-fold) and TIMP-1 (4-fold) in THP1-CD14 monocytes, as well as the gelatinolytic activity of MMP-9. Co-incubation of monocytes with LDL(?) and SDX for 24?h significantly reduced both the release of MMP-9 and TIMP-1 and gelatinase activity. In THP1 cells not expressing CD14, no effect of LDL(?) on MMP-9 or TIMP-1 release was observed. The uptake of DiI-labeled LDL(?) was higher than that of DiI-LDL(+) in THP1-CD14 but not in THP1 cells. This increase was inhibited by SDX. Experiments in microtiter wells coated with SDX demonstrated a specific interaction of LDL(?) with SDX.Conclusions
LDL(?) induced the release of MMP-9 and TIMP-1 in monocytes through CD14. SDX affects the ability of LDL(?) to promote TIMP-1 and MMP-9 release by its interaction with LDL(?). 相似文献60.
《Cytokine》2016
Suppressor of cytokine signaling 1 (SOCS1) is an indispensable regulator of IFNγ signaling and has been implicated in the regulation of liver fibrosis. However, it is not known whether SOCS1 mediates its anti-fibrotic functions in the liver directly, or via modulating IFNγ, which has been implicated in attenuating hepatic fibrosis. Additionally, it is possible that SOCS1 controls liver fibrosis by regulating hepatic stellate cells (HSC), a key player in fibrogenic response. While the activation pathways of HSCs have been well characterized, the regulatory mechanisms are not yet clear. The goals of this study were to dissociate IFNγ-dependent and SOCS1-mediated regulation of hepatic fibrogenic response, and to elucidate the regulatory functions of SOCS1 in HSC activation. Liver fibrosis was induced in Socs1−/−Ifng−/− mice with dimethylnitrosamine or carbon tetrachloride. Ifng−/− and C57BL/6 mice served as controls. Following fibrogenic treatments, Socs1−/−Ifng−/− mice showed elevated serum ALT levels and increased liver fibrosis compared to Ifng−/− mice. The latter group showed higher ALT levels and fibrosis than C57BL/6 controls. The livers of SOCS1-deficient mice showed bridging fibrosis, which was associated with increased accumulation of myofibroblasts and abundant collagen deposition. SOCS1-deficient livers showed increased expression of genes coding for smooth muscle actin, collagen, and enzymes involved in remodeling the extracellular matrix, namely matrix metalloproteinases and tissue inhibitor of metalloproteinases. Primary HSCs from SOCS1-deficient mice showed increased proliferation in response to growth factors such as HGF, EGF and PDGF, and the fibrotic livers of SOCS1-deficient mice showed increased expression of the Pdgfb gene. Taken together, these data indicate that SOCS1 controls liver fibrosis independently of IFNγ and that part of this regulation may occur via regulating HSC proliferation and limiting growth factor availability. 相似文献